Reagents and BuffersClick the links for details of the reagents and buffers used for the applications described on this website. |
| Reagent | for the measurement of... |
|---|---|
|
Benedict's solution
and Quantitative Benedict's solution |
Reducing sugars |
| Biuret solution | Protein |
| Bradford reagent | Protein |
| DNSA | Reducing sugars |
| Iodine solution | various |
| Buffer | pH range |
|---|---|
| Phosphate 0.1M | 5.8-8.0 |
| Citric acid-Sodium phosphate | 2.6-7.6 |
|
Citric acid-Sodium citrate 0.1M Phosphate free |
3.0-6.2 |
| Low pH buffer | 1.0-2.2 |
| High pH buffer | 9.2-10.8 |