AQA Biology Practical 11

Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown 'urine' sample.

The AQA handbook suggests either qualitative or quantitative Benedict's solution for this practical.
The handbook recommends using a colorimeter such as WPA with filters for 600nm and 680nm light. The Mystrica colorimeter uses a red LED (~630nm) for both quantitative and qualitative Benedict's. Comparative results with the two colorimeters shown here illustrate that perfectly good results can be obtained with either.

Both types of Benedict's solution produce precipitates which have to be removed, by filtering, centrifuging or settling, before accurate results can be obtained with a colorimeter.

An alternative method uses DNSA. This method has a lot to recommend it. It is simpler as there is no precipitate involved, and it is able to detect lower concentrations of glucose than are possible with Benedict's solution. In addition to the information on this website there is a good piece on the NCBE website and CLEAPSS has information.