Citric acid-Sodium citrate buffer 0.1MPhosphate free buffer
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Prepare a 0.1M solution of citric acid monohydrate, C6H8 O7·H2O (21.01g/l) and a 0.1M solution of trisodium citrate dihydrate,
C6H5 O7Na3·2H2O (29.41g/l)
Mix the volumes shown in the table.
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| pH |
0.1M citric acid (cm3)
|
0.1M trisodium citrate (cm3)
|
| 3.0 |
82.0 |
18.0 |
| 3.2 |
77.5 |
22.5 |
| 3.4 |
73.0 |
27.0 |
| 3.6 |
68.5 |
31.5 |
| 3.8 |
63.5 |
36.5 |
| 4.0 |
59.0 |
41.0 |
| 4.2 |
54.0 |
46.0 |
| 4.4 |
49.5 |
50.5 |
| 4.6 |
44.5 |
55.5 |
| 4.8 |
40.0 |
60.0 |
| 5.0 |
35.0 |
65.0 |
| 5.2 |
30.5 |
69.5 |
| 5.4 |
25.5 |
74.5 |
| 5.6 |
21.0 |
79.0 |
| 5.8 |
16.0 |
84.0 |
| 6.0 |
11.5 |
88.5 |
| 6.2 |
8.0 |
92.0 |
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